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SNI ISO 16649-1:2016 : Mikrobiologi bahan pangan dan pakan - metode horizontal enumerasi B-glucuronidase- escherichia coli positif bagian 1: teknik penghitungan koloni menggunakan membran pada suhu 44 °C dan 5-bromo-4-chloro-3-indolyl B-D-glucuronide (ISO 16649.1:2001, IDT Eng)
This part of ISO 16649 specifies a horizontal method for the enumeration of b-glucuronidase-positive Escherichia coli in products intended for human consumption or for the feeding of animals. It uses a colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme b-glucuronidase.
WARNING Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are B-glucuronidase negative, such as Escherichia coli 0157, will not be detected.
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