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SNI ISO 16649-3:2016 : Mikrobiologi rantai pangan - metode horizontal enumerasi ẞ-glucuronidase-positive escherichia coli bagian 3: deteksi dan penentuan angka paling mungkin (APN) menggunakan 5-bromo-4-chloro-3-indolyl-B-D-glucuronide (ISO 16649.3:2015, IDT Eng)
This part of ISO 16649 specifies a horizontal method for the detection and enumeration of B-glucuronidase positive by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This part of ISO 16649 is Escherichia coli applicable to the following:
products intended for human consumption and the feeding of animals:
environmental samples in the area of food production and food handling.
The method is suitable for the enumeration of cells of that might have been subjected to stress arising from dehydration, freezing, and exposure to a saline E.coli (such as marine) environment or damage by disinfectants such as chlorine-containing products.
A limitation of the applicability of this part of ISO 16649 is imposed by the susceptibility of the method to a large degree of variability The method is intended to be applied and the results interpreted in the light of the information given in Clause 11
This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO 7251 is intended to be used for milk and milk products.
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